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Related Experiment Video

Updated: May 7, 2026

A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish

A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish

Published on: March 19, 2015

FISH on Histological Sections.

Irina Solovei¹, Florian Grasser, Christian Lanctôt

¹Ludwig-Maximilians University Munich, Department of Biology II, AG Thomas Cremer (Chair of Anthropology and Human Genetics), 82152 Martinsried-Planegg, Germany.

|March 2, 2011

Summary

This study details fluorescence in situ hybridization (FISH) for visualizing DNA targets in tissue sections. The method preserves nuclear structure for studying the genome's native 3D organization.

Area of Science:

Molecular Biology
Genomics
Cell Biology

Background:

Visualizing specific DNA targets within tissue organization is crucial for understanding genome function.
Existing methods may not adequately preserve the native 3D nuclear structure.

Purpose of the Study:

To describe a detailed protocol for fluorescence in situ hybridization (FISH) on histological sections.
To enable visualization of DNA targets, including chromosome territories and subregions, within functional tissue context.
To ensure preservation of nuclear morphology for studying the native 3D genome structure.

Main Methods:

Developed protocols for FISH on paraffin-embedded, vibratome, or frozen tissue sections.
Included pretreatment steps (heat or protease) for DNA target unmasking and reagent penetration.

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Emphasized techniques to maintain native nuclear morphology.

Main Results:

Successfully visualized specific DNA targets within histological sections.
Demonstrated the ability to study chromosome territories and subregions in the context of tissue organization.
Validated protocols for different tissue preparation methods.

Conclusions:

Fluorescence in situ hybridization (FISH) is an effective method for visualizing DNA targets in histological sections.
The described protocols allow for the study of the native 3D genome structure by preserving nuclear morphology.
This technique provides valuable insights into genome organization within functional tissue contexts.