Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fc-GDF15 glyco-engineering and receptor binding affinity optimization for body weight regulation.

Scientific reports·2021
Same author

Cultivating social relationships and disrupting social isolation in low-income, high-disparity neighbourhoods in Ohio, USA.

Health & social care in the community·2021
Same author

Discovery and optimization of a novel anti-GUCY2c x CD3 bispecific antibody for the treatment of solid tumors.

mAbs·2021
Same author

Fluorescent Western Blotting: High Sensitivity Detection of Multiple Targets.

Current protocols in pharmacology·2020
Same author

In-gel peptide IEF sample preparation for LC/MS analysis.

Methods in molecular biology (Clifton, N.J.)·2015
Same author

2-D Western blotting for evaluation of antibodies developed for detection of host cell protein.

Methods in molecular biology (Clifton, N.J.)·2015

Related Experiment Video

Updated: Jun 4, 2026

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
10:27

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Published on: April 20, 2018

Mass spectrometry-compatible silver staining.

Wayne R Stochaj, Tom Berkelman, Nancy Laird

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary
    This summary is machine-generated.

    A new silver staining method allows protein detection on gels for mass spectrometry identification. While less sensitive than standard methods, it avoids protein modification, enabling compatibility with techniques like MALDI and ESI-MS.

    More Related Videos

    Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
    06:24

    Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

    Published on: April 21, 2019

    Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues
    07:05

    Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

    Published on: October 4, 2018

    Related Experiment Videos

    Last Updated: Jun 4, 2026

    A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
    10:27

    A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

    Published on: April 20, 2018

    Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
    06:24

    Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

    Published on: April 21, 2019

    Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues
    07:05

    Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

    Published on: October 4, 2018

    Area of Science:

    • Biochemistry
    • Proteomics
    • Analytical Chemistry

    Background:

    • Standard ammoniacal silver staining is sensitive for protein detection on SDS-PAGE gels.
    • Traditional silver staining methods are incompatible with mass spectrometry (MS) due to protein modification.
    • Mass spectrometry is crucial for identifying proteins separated on 2D gels.

    Purpose of the Study:

    • To develop a silver staining method compatible with mass spectrometry.
    • To enable protein identification after gel separation without compromising MS analysis.

    Main Methods:

    • A modified silver staining protocol was employed.
    • The method avoids chemical modifications incompatible with mass spectrometry.
    • Compatibility with Matrix-Assisted Laser Desorption/Ionization (MALDI) and Electrospray Ionization (ESI)-MS was assessed.

    Main Results:

    • The developed method is compatible with both MALDI and ESI-MS.
    • It handles semipreparative protein loads effectively without negative staining.
    • The staining sensitivity is lower compared to standard silver staining methods.

    Conclusions:

    • This novel silver staining technique facilitates direct protein identification by mass spectrometry.
    • It offers a valuable alternative for proteomic studies requiring MS analysis of gel-separated proteins.