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Published on: October 10, 2011
Staining mouse embryos for alkaline phosphatase activity
CSH Protocols
|March 2, 2011
Abstract:
This protocol describes a technique that is used to visualize human placental alkaline phosphatase (AP) activity in transgenic mice. AP activity staining is localized to the cell surface. Endogenous AP activity is eliminated by heat inactivation that spares the heat-stable human placental AP activity.
