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Related Concept Videos

The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
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B Cell Activation and Differentiation

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General Transcription Factors

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Related Experiment Video

Updated: Jun 4, 2026

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

Stat6 and c-Jun mediate Th2 cell-specific IL-24 gene expression.

Anupama Sahoo1, Choong-Gu Lee, Arijita Jash

  • 1School of Life Sciences and Immune Synapse Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.

Journal of Immunology (Baltimore, Md. : 1950)
|March 2, 2011
PubMed
Summary

T-cell receptor (TCR) stimulation induces Interleukin-24 (IL-24) gene expression in Th2 cells. This process involves the synergistic action of Stat6 and c-Jun transcription factors on the IL-24 promoter.

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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

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Last Updated: Jun 4, 2026

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Area of Science:

  • Immunology
  • Molecular Biology
  • Gene Regulation

Background:

  • T-cell receptor (TCR) signaling is crucial for T cell function and cytokine gene expression.
  • Interleukin-24 (IL-24), an IL-10 family cytokine, has diverse roles including anticancer effects and regulation of immune pathology.
  • IL-24 is selectively expressed in activated Th2 cells upon TCR stimulation, but the underlying molecular mechanisms are not fully understood.

Purpose of the Study:

  • To elucidate the molecular mechanisms driving Th2 cell-specific expression of the IL-24 gene.
  • To identify key regulatory elements and transcription factors involved in IL-24 gene induction by TCR signaling.

Main Methods:

  • Identification and characterization of the proximal promoter region (-157/+95 bp) of the mouse IL-24 gene.
  • Analysis of chromatin structure and histone modifications at the IL-24 promoter in Th2 cells.
  • Investigation of in vivo binding of transcription factors Stat6 and AP-1 (c-Jun) to the IL-24 promoter.
  • Assessment of the synergistic effects of Stat6 and c-Jun on IL-24 promoter activity.
  • Evaluation of IL-24 gene expression following knockdown of Stat6 or c-Jun in Th2 cells.

Main Results:

  • A critical proximal promoter region (-157/+95 bp) was identified for IL-24 gene activation in Th2 cells.
  • This promoter region exhibits Th2 cell-specific open chromatin structure and permissive histone modifications.
  • Stat6 and c-Jun were found to bind to the IL-24 promoter in vivo and synergistically transactivate its expression.
  • Physical cooperation between Stat6 and c-Jun proteins was observed, leading to increased IL-24 gene transcription.
  • Knockdown of either Stat6 or c-Jun significantly suppressed endogenous IL-24 gene expression in Th2 cells.

Conclusions:

  • TCR stimulation induces IL-24 expression in Th2 cells through the coordinated action of Stat6 and c-Jun transcription factors.
  • These transcription factors regulate IL-24 expression at the transcriptional level via interaction with its proximal promoter.
  • The findings provide novel insights into the molecular regulation of IL-24 in Th2 immune responses.