Related Experiment Video
Updated: Jun 4, 2026

Aip1p Dynamics Are Altered by the R256H Mutation in Actin
Published on: July 30, 2014
Structure of the sortase AcSrtC-1 from Actinomyces oris
1Department of Odontology, Umeå University, Umeå, Sweden. karina.persson@odont.umu.se
The crystal structure of Actinomyces oris sortase AcSrtC-1 was determined, revealing its role in fimbriae formation. Calcium enhances the enzyme
Area of Science:
- Structural Biology
- Microbiology
- Biochemistry
Background:
- Actinomyces oris is an oral microorganism.
- Sortases are enzymes crucial for bacterial virulence and cell wall anchoring.
- AcSrtC-1 is a sortase involved in fimbriae formation in A. oris.
Purpose of the Study:
- To determine the crystal structure of AcSrtC-1.
- To understand the mechanism of fimbriae polymerization mediated by AcSrtC-1.
- To investigate the structural features of the AcSrtC-1 active site.
Main Methods:
- X-ray crystallography to determine the 2.4 Å resolution crystal structure of AcSrtC-1.
- Analysis of the enzyme's active site conformation.
- Assessment of enzyme thermostability in the presence of calcium.
Main Results:
- The crystal structure of AcSrtC-1 was solved to 2.4 Å resolution.
- The enzyme's active site, a cysteine-histidine-arginine triad, was observed in two distinct conformations.
- A flexible lid protects the active site, typical of pili-associated sortases.
- Calcium ions were found to increase the thermostability of AcSrtC-1.
Conclusions:
- The determined structure provides insights into the mechanism of fimbriae assembly.
- Understanding AcSrtC-1 structure and function can aid in developing targeted antimicrobial strategies.
- Calcium's role in enhancing thermostability suggests potential applications in enzyme engineering.
More Related Videos
12:15The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
Published on: October 3, 2017
12:15In Vitro Polymerization of F-actin on Early Endosomes
Published on: August 28, 2017
Related Concept Videos
Introduction to Actin
Formation of Higher-order Actin Filaments
The high-order actin networks...
Overview of Myosin Structure and Function
Bacterial Phylum Actinobacteria
The Role of Actin and Myosin in Non-muscle Cells
Generation of Straight or Branched Actin Filaments
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...