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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

Updated: Jun 4, 2026

Generating Primary Cultures for Keratinocyte Live Cell Imaging

Generating Primary Cultures for Keratinocyte Live Cell Imaging

Published on: May 2, 2025

Keratocyte cell culture.

N R Richard¹, R W Lambert, J A Anderson

¹National Vision Research Institute, San Diego, CA.

Methods in Molecular Medicine

|March 2, 2011

Summary

This summary is machine-generated.

Corneal fibroblasts, known as keratocytes, are quiescent in healthy eyes but activate and repair damage after injury. This cellular response is crucial for maintaining corneal tissue integrity.

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Area of Science:

Ophthalmology
Cell Biology
Tissue Engineering

Background:

Keratocytes are the primary cells within the corneal stroma.
These cells form an interconnected network, potentially involved in tissue-wide communication.
Normally, adult corneal keratocytes are quiescent but responsive to injury.

Purpose of the Study:

To describe the role and behavior of keratocytes in corneal tissue.
To highlight the regenerative capacity of keratocytes following injury.

Main Methods:

Review of existing literature on corneal fibroblast biology.
Analysis of cellular responses to simulated or actual corneal trauma.

Main Results:

Keratocytes are quiescent under normal conditions.
Upon injury, keratocytes near the affected area activate and proliferate.
Activated keratocytes synthesize and replace damaged stromal matrix components.

Conclusions:

Keratocytes are essential for corneal wound healing and matrix repair.
Their differentiation into active cells is a key response to corneal trauma.