Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting obesity and lipid metabolism profiles to prevent perianal abscesses: A case-control study and Mendelian randomization analysis.

World journal of gastrointestinal surgery·2026

Same author

Polymerization-mediated SRFR1 condensation in upper lateral root cap cells regulates root growth.

The Plant cell·2025

Same author

Complete blood counts and their derived inflammatory markers with anal fistulas in male children: A retrospective case-control study.

World journal of gastroenterology·2025

Same author

Gating of immune-triggered cell death is a redox rhythm-specific output.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Relationship between serum iron, zinc, calcium, and HIF-1a-comparative analysis of 2 regions and 4 ethnic groups in China.

Frontiers in nutrition·2024

Same author

The processed C-terminus of AvrRps4 effector suppresses plant immunity via targeting multiple WRKYs.

Journal of integrative plant biology·2024

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Quantifying alternatively spliced mRNA via capillary electrophoresis.

Xue-Cheng Zhang¹, Walter Gassmann

¹Division of Plant Sciences, Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 2, 2011

Summary

Researchers developed a novel method to quantify alternative splicing (AS) transcripts in plants. This technique accurately measures AS variants of low-expressed genes, like the RPS4 resistance gene, during immune responses.

More Related Videos

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Related Experiment Videos

Last Updated: Jun 4, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

Plant molecular biology
Gene expression analysis
Molecular genetics

Background:

Alternative splicing (AS) increases transcriptome and proteome complexity in plants, with ~22% of genes undergoing AS.
The functional significance of individual AS products remains largely unexplored in plants.
The Arabidopsis RPS4 resistance gene requires AS transcripts for its function.

Purpose of the Study:

To develop a method for quantifying alternative splicing transcripts.
To address limitations of existing methods for low-expressed genes with complex splicing patterns.
To analyze differential regulation of RPS4 alternative transcripts during effector-triggered immunity.

Main Methods:

Developed a novel method combining reverse transcription PCR (RT-PCR) and capillary electrophoresis.
RT-PCR amplified all transcript variants using common primers.
Quantified products using signal quantification procedures for microsatellite genotyping on capillary sequencing machines.

Main Results:

Successfully quantified individual RPS4 alternative transcripts.
Demonstrated differential regulation of RPS4 AS transcripts during effector-triggered immunity.
Validated the method's suitability for low-expressed genes with complex splicing.

Conclusions:

The developed method enables accurate quantification of alternative splicing transcripts.
This technique is particularly valuable for studying low-expressed genes with complex splicing patterns.
Provides insights into the regulation of alternative splicing in plant immune responses.