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Related Experiment Video

Updated: Jun 4, 2026

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
07:14

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

Published on: August 27, 2010

Assaying effector function in planta using double-barreled particle bombardment.

Shiv D Kale1, Brett M Tyler

  • 1Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 2, 2011
PubMed
Summary

Researchers developed a double-barreled gene gun for biolistic transient gene expression assays. This innovation significantly improves accuracy and reduces experimental replicates for studying gene function in vivo.

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Area of Science:

  • Plant molecular biology
  • Gene expression analysis
  • Biotechnology

Background:

  • Biolistic transient gene expression assays are vital for in vivo gene function studies.
  • Conventional methods suffer from inaccuracies like poor transformation efficiency, potentially masking biological effects.

Purpose of the Study:

  • To introduce an improved biolistic assay system for enhanced accuracy and efficiency.
  • To reduce the number of experimental replicates needed for statistically significant results.

Main Methods:

  • Development and application of a novel double-barreled gene gun attachment.
  • Simultaneous biolistic delivery of control and test DNA preparations onto the same leaf.
  • Calculating gene activity by the ratio of test to control bombardment results.

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Last Updated: Jun 4, 2026

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
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A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

Published on: August 27, 2010

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
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Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
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Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

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Main Results:

  • The double-barreled device achieves statistically significant data with one-tenth the replicates of conventional assays.
  • The ratio of test to control bombardments provides a reproducible measure of gene activity.
  • Successfully applied to study plant resistance gene responses, cell death modulation, and oomycete effector functions.

Conclusions:

  • The double-barreled gene gun attachment significantly enhances the reliability and efficiency of biolistic transient gene expression assays.
  • This method provides a robust tool for accurate gene function studies, particularly in plant science.
  • Enables precise quantification of gene activity and reduces experimental variability.