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Immunolocalization of pathogen effectors

Eric Kemen1, Kurt Mendgen, Ralf T Voegele

  • 1The Sainsbury Laboratory, John Innes Centre, Norwich, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 2, 2011
PubMed

The use of polyclonal antibodies enables the detection of proteins on a cellular and even subcellular level. Immunolocalization can be used on all pathosystems even if one or both partners of the interaction are unamenable to molecular tools like transformation. This chapter provides detailed information about how to obtain high quality antibodies, how to prepare samples, and finally how to detect the proteins. Methods for light and electron microscopy are presented.

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