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Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.

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Related Experiment Video

Updated: Jun 4, 2026

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

DMSO regulates osteoclast development in vitro.

Justin M Lemieux1, Gary Wu, Joseph A Morgan

  • 1Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, CT, USA.

In Vitro Cellular & Developmental Biology. Animal
|March 2, 2011
PubMed
Summary
This summary is machine-generated.

Dimethyl sulfoxide (DMSO) significantly impacts osteoclast development. Researchers should exercise caution, as DMSO concentration variations can profoundly affect experimental outcomes, particularly in osteoclast differentiation studies.

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Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

Related Experiment Videos

Last Updated: Jun 4, 2026

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

Area of Science:

  • Cell Biology
  • Skeletal Biology
  • Pharmacology

Background:

  • Dimethyl sulfoxide (DMSO) is a common laboratory solvent and vehicle.
  • Previous studies have involved DMSO with myeloid cells and macrophages.
  • Data on DMSO's isolated effects on osteoclast development is lacking.

Purpose of the Study:

  • To investigate the direct effects of DMSO on osteoclast differentiation.
  • To quantify changes in osteoclast morphology and cellularity induced by DMSO.
  • To highlight potential confounding effects of DMSO in osteoclast research.

Main Methods:

  • Osteoclasts were generated from bone marrow macrophages and an osteoclast progenitor cell line.
  • Cells were cultured with varying concentrations and durations of DMSO.
  • Osteoclast development was assessed by measuring cell number, surface area, and nuclei count.

Main Results:

  • A 5 microM concentration of DMSO increased osteoclast surface area significantly (from 38,510 to 156,422 pixels).
  • A 10 microM concentration of DMSO decreased osteoclast surface area (to 18,994 pixels).
  • Serial addition and removal of DMSO demonstrated dose-dependent effects on osteoclast surface area and nuclei count.

Conclusions:

  • DMSO exerts a marked and concentration-dependent effect on osteoclast differentiation.
  • Variations in DMSO concentrations can significantly alter experimental data in osteoclast studies.
  • Caution is advised when using DMSO as a vehicle in studies assessing osteoclast differentiation.