Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 4, 2026

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
14:04

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Published on: January 16, 2021

SAD phasing using iodide ions in a high-throughput structural genomics environment.

Jan Abendroth1, Anna S Gardberg, John I Robinson

  • 1Emerald BioStructures, 7869 NE Day Road West, Bainbridge Island, WA 98110, USA.

Journal of Structural and Functional Genomics
|March 2, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Trichomonas vaginalis</i> PPi-dependent phosphofructokinase structures reveal a non-canonical nucleotide binding site.

bioRxiv : the preprint server for biology·2026
Same author

Dual-Mode Native Mass Spectrometry Screening Identifies Ginsenoside Ligands of 6-Hydroxymethyl-7,8-Dihydropterin Pyrophosphokinase (HPPK).

Molecules (Basel, Switzerland)·2026
Same author

Assessing Metal Ion Assignment Accuracy in Protein Data Bank Models via Elemental Spectroscopy.

Journal of chemical information and modeling·2026
Same author

Crystal structure of Plasmodium vivax macrophage migration inhibitory factor.

Acta crystallographica. Section F, Structural biology communications·2026
Same author

Automated extraction and optimization of protein purification protocols using multi-agent large language models.

bioRxiv : the preprint server for biology·2026
Same author

Structural and Functional Analyses of <i>Trypanosoma brucei</i> Nucleoside Diphosphate Kinase.

ACS omega·2026

Structural genomics uses iodide ion single wavelength anomalous dispersion (SAD) phasing for rapid, low-cost determination of infectious disease targets. This method achieved a 94% success rate, enabling structure elucidation for novel proteins.

Area of Science:

  • Structural biology
  • Infectious disease research
  • Drug target identification

Background:

  • The Seattle Structural Genomics Center for Infectious Disease (SSGCID) targets proteins from infectious organisms for drug discovery.
  • Many targets are amenable to molecular replacement (MR) due to known homologous structures.
  • However, some targets lack homologs or do not solve well with MR, requiring experimental phasing.

Purpose of the Study:

  • To present iodide ion single wavelength anomalous dispersion (SAD) as a primary de novo phasing method.
  • To demonstrate the efficiency and broad applicability of iodide SAD phasing in a high-throughput structural genomics setting.
  • To showcase its utility for proteins with novel folds and weak MR solutions.

Main Methods:

  • Utilizing iodide ion soaks for single wavelength anomalous dispersion (SAD) experiments.

More Related Videos

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

Microcrystallography of Protein Crystals and In Cellulo Diffraction
09:35

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

Related Experiment Videos

Last Updated: Jun 4, 2026

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
14:04

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Published on: January 16, 2021

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

Microcrystallography of Protein Crystals and In Cellulo Diffraction
09:35

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

  • Employing native crystals and in-house data collection for rapid structure determination.
  • Applying SAD phasing for de novo phase determination and combined with MR for challenging targets.

    • Main Results:

    • Achieved a 94% success rate (16 out of 17 cases) in structure determination within the first year of adopting iodide SAD phasing.
    • Successfully elucidated structures of proteins with novel folds using SAD phasing.
    • Demonstrated successful structure determination for targets with weak MR solutions by combining SAD and MR.

    Conclusions:

    • Iodide ion SAD phasing is a rapid, low-cost, and highly effective method for de novo phasing in structural genomics.
    • This technique is versatile and applicable across various crystallization conditions and protein types.
    • Iodide SAD phasing significantly enhances the structure elucidation capabilities for infectious disease drug targets.