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Updated: Jun 4, 2026

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

SWAP70, actin-binding protein, function as an oncogene targeting tumor-suppressive miR-145 in prostate cancer

Takeshi Chiyomaru1, Shuichi Tatarano, Kazumori Kawakami

  • 1Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.

The Prostate
|March 2, 2011
PubMed
Abstract

Insights

MicroRNA-145 (miR-145) down-regulation in prostate cancer (CaP) suggests a tumor suppressive role. This study identifies SWAP70 as a direct target of miR-145, potentially acting as an oncogene in CaP progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-145 (miR-145) is frequently downregulated in various human cancers, including prostate cancer (CaP).
  • Previous research indicated that miR-145 targets actin-binding proteins in bladder and prostate cancer.
  • This study aimed to identify a novel oncogenic gene targeted by miR-145 within the actin-binding protein family in CaP.

Purpose of the Study:

  • To determine if SWAP70 (SWAP switching B-cell complex 70 kDa subunit) is a direct target of miR-145.
  • To investigate the functional role of SWAP70 in prostate cancer cell behavior.
  • To assess SWAP70 expression levels in clinical prostate cancer specimens.

Main Methods:

  • Utilized luciferase reporter assays to confirm direct binding between miR-145 and SWAP70 mRNA.
  • Performed cell viability assays, including proliferation, wound healing, and matrigel invasion, following SWAP70 silencing (si-SWAP70).
  • Conducted immunohistochemistry for SWAP70 on 75 clinical prostate cancer specimens and benign prostate hyperplasia tissues.

Main Results:

  • Luciferase assays confirmed SWAP70 as a direct molecular target regulated by miR-145.
  • Silencing SWAP70 significantly inhibited cell migration and invasion in prostate cancer cell lines.
  • SWAP70 expression was significantly higher in prostate cancer tissues compared to benign prostate hyperplasia.

Conclusions:

  • Downregulation of miR-145 in CaP suggests a tumor suppressive function.
  • SWAP70 is identified as a potential oncogenic target of miR-145, contributing to CaP progression.
  • The miR-145/SWAP70 axis offers novel insights into the molecular mechanisms underlying CaP.
Keywords:
SWAP70actin-binding proteinmiR-145microRNAprostate cancer

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