A two-dimensional polarization interferometry based parallel scan angular surface plasmon resonance biosensor

We describe a two-dimensional polarization interferometry based parallel scan angular surface plasmon resonance (SPR) biosensing technique. The method of line-shaped light illumination and parallel scan offers a high throughput. The simultaneous record of SPR angular spectrum enables the system to be unaffected by the time-dependent variation of the light source. The polarization interferometry technique lowers the minimum of the SPR dip and thereby reduces the noise related to the light intensity. Refractive index resolutions of 1.4 × 10(-6) refractive index unit (RIU) under normal condition and 4.6 × 10(-7) RIU under a more time-consuming condition are achieved in our angle interrogation based sensor. Meanwhile, a manually prepared DNA microarray has been detected, showing the potential applications of this technique in microarray analysis.