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A two-dimensional polarization interferometry based parallel scan angular surface plasmon resonance biosensor.

Le Liu¹, Suihua Ma, Yanhong Ji

¹Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China.

The Review of Scientific Instruments

|March 3, 2011

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A novel two-dimensional polarization interferometry technique enhances surface plasmon resonance (SPR) biosensing for high-throughput analysis. This method achieves high refractive index resolution, demonstrating potential for DNA microarray detection.

Area of Science:

Optics and Photonics
Biotechnology
Nanotechnology

Background:

Surface Plasmon Resonance (SPR) is a label-free optical technique widely used for biosensing.
Traditional SPR methods can be limited by throughput and sensitivity to environmental fluctuations.
Improving SPR sensitivity and stability is crucial for advanced biomolecular detection.

Purpose of the Study:

To develop a high-throughput, stable, and sensitive two-dimensional (2D) SPR biosensing technique.
To enhance the signal-to-noise ratio and refractive index resolution of SPR measurements.
To demonstrate the applicability of the developed technique for DNA microarray analysis.

Main Methods:

Utilized a two-dimensional polarization interferometry setup for parallel scan angular SPR.

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Last Updated: Jun 4, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

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Published on: July 21, 2018

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Published on: March 17, 2023

Employed line-shaped light illumination for high-throughput data acquisition.

Simultaneously recorded SPR angular spectra to mitigate light source variations.

Applied polarization interferometry to minimize SPR dip noise.

Main Results:

Achieved a refractive index resolution of 1.4 × 10⁻⁶ RIU under normal conditions.
Attained a higher resolution of 4.6 × 10⁻⁷ RIU under optimized, time-consuming conditions.
Successfully detected a manually prepared DNA microarray, validating the technique's potential.

Conclusions:

The developed 2D polarization interferometry SPR biosensing technique offers high throughput and enhanced sensitivity.
The method demonstrates robustness against light source fluctuations and reduced noise.
This technique shows significant promise for applications in microarray analysis and other high-resolution biosensing fields.