Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Feasibility of chin wing osteotomy: Can it be considered a routine procedure in orthognathic surgery?

Journal of stomatology, oral and maxillofacial surgery·2025
Same author

The financial burden of healthcare-associated infections: a propensity score analysis in an Italian healthcare setting.

Infection prevention in practice·2025
Same author

A versatile system for the growth of porphyrin films via electrospray and molecular sublimation in vacuum and their multi-technique characterization.

The Review of scientific instruments·2024
Same author

Coherent control of rare earth 4f shell wavefunctions in the quantum spin liquid Tb<sub>2</sub>Ti<sub>2</sub>O<sub>7</sub>.

Nature communications·2024
Same author

Not only COVID-19: a systematic review of anti-COVID-19 measures and their effect on healthcare-associated infections.

The Journal of hospital infection·2024
Same author

Investigating the activation of passive metals by a combined in-situ AFM and Raman spectroscopy system: a focus on titanium.

Scientific reports·2023
Same journal

Compressed multi-scale entropy and its application in mechanical fault diagnosis.

The Review of scientific instruments·2026
Same journal

Bidirectional drive and multi-resolution adjustment across frequency bands in inertial impact piezoelectric motors via multimodal resonant vibration.

The Review of scientific instruments·2026
Same journal

A magnetic field sensor based on flaky Terfenol-D material and dual fiber grating.

The Review of scientific instruments·2026
Same journal

A novel E-field eight-way cavity combiner for high-power S-band applications.

The Review of scientific instruments·2026
Same journal

Constant radius blade spring suspended bench for vibration isolation.

The Review of scientific instruments·2026
Same journal

Qualification of infrared optical fibers and emitters for a spectrometer for in situ planetary exploration: Results from the TRIS (TRansmission and Illumination System) project.

The Review of scientific instruments·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2026

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human &alpha;-actinin by Confocal Imaging
12:58

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging

Published on: October 24, 2010

Apparatus for vectorial Kerr confocal microscopy.

M Savoini1, F Ciccacci, L Duò

  • 1CNISM-Dipartimento di Fisica, Politecnico di Milano, Milano, Italy.

The Review of Scientific Instruments
|March 3, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel confocal microscopy technique to measure magnetic hysteresis loops. This method separates in-plane and out-of-plane magnetization components with high spatial resolution.

More Related Videos

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
07:40

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

Published on: August 9, 2019

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
13:08

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Published on: October 7, 2010

Related Experiment Videos

Last Updated: Jun 4, 2026

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human &alpha;-actinin by Confocal Imaging
12:58

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging

Published on: October 24, 2010

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
07:40

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

Published on: August 9, 2019

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
13:08

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Published on: October 7, 2010

Area of Science:

  • Magnetism and optical physics
  • Advanced microscopy techniques
  • Materials characterization

Background:

  • Magneto-optical effects are crucial for studying magnetic materials.
  • Accurate characterization of magnetization components is essential for materials science.
  • Existing microscopy methods may have limitations in resolving magnetization directions.

Purpose of the Study:

  • To present a new confocal microscopy setup for magneto-optical hysteresis measurements.
  • To enable the separation of in-plane and out-of-plane magnetization components.
  • To achieve high-resolution magneto-optical imaging.

Main Methods:

  • Modification of a commercial confocal microscope.
  • Deviation of the light beam from the objective's optical axis using a translating plate.
  • Utilizing magneto-optical Kerr microscopy principles.

Main Results:

  • Successful recording of magneto-optical hysteresis cycles.
  • Demonstrated ability to distinguish between in-plane and out-of-plane magnetization.
  • Achieved a lateral resolution of 600 nm at a wavelength of 635 nm.

Conclusions:

  • The developed setup provides a powerful tool for analyzing magnetic properties.
  • This technique enhances the understanding of magnetic materials at the nanoscale.
  • The method offers improved capabilities for magneto-optical imaging and analysis.