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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 4, 2026

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Ultracompact alignment-free single molecule fluorescence device with a foldable light path.

Niraj Kumar Singh1, Jenu V Chacko, Varun K A Sreenivasan

  • 1Tata Institute of Fundamental Research, Homi Bhaba Road, Colaba, Mumbai 400005, India.

Journal of Biomedical Optics
|March 3, 2011
PubMed
Summary
This summary is machine-generated.

We developed a compact, alignment-free single-molecule detection instrument using fiber optics. This portable device offers high resolution for potential medical diagnostics and field applications.

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Conducting Multiple Imaging Modes with One Fluorescence Microscope
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Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

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Last Updated: Jun 4, 2026

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

  • Biophysics
  • Optical Engineering
  • Medical Diagnostics

Background:

  • Single-molecule detection instruments are valuable but often large, costly, and complex.
  • Existing fiber-only devices lack the resolution and sensitivity of larger systems and require precise alignment.

Purpose of the Study:

  • To develop a compact, alignment-free instrument for single-molecule detection.
  • To retain high resolution and sensitivity in a significantly smaller device.

Main Methods:

  • Utilized through-space reciprocal optical coupling between a fiber and a microscope objective.
  • Implemented wavelength division multiplexing in optical fibers.
  • Developed a 4 × 4 × 18 cm³ fluorescence correlation spectrometer.

Main Results:

  • Achieved single-molecule level kinetic analysis with optical resolution comparable to larger microscope-based systems.
  • The device requires no alignment, drastically reducing size and complexity.
  • Sensitivity is theoretically comparable, though practically limited by fiber background fluorescence.

Conclusions:

  • A portable, field-deployable single-molecule device with practical diagnostic applications has been demonstrated.
  • The developed technology significantly reduces the size and complexity of single-molecule detection instruments.
  • Further improvements in fiber technology could enhance sensitivity for broader applications.