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Related Experiment Video

Updated: Jun 4, 2026

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Optical transfection using an endoscope-like system.

Nan Ma1, Frank Gunn-Moore, Kishan Dholakia

  • 1University of St. Andrews, School of Physics & Astronomy, St. Andrews, Fife KY16 9SS United Kingdom. nm210@st-andrews.ac.uk

Journal of Biomedical Optics
|March 3, 2011
PubMed
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This summary is machine-generated.

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Researchers developed an endoscope-like system for optical transfection, achieving high plasmid delivery efficiency in cells. This innovation enables precise, targeted delivery of genetic material for potential therapeutic applications.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Optical Physics

Background:

  • Optical transfection utilizes focused laser beams to temporarily increase cell membrane permeability, facilitating the entry of foreign genetic material.
  • Existing methods often lack the precision and flexibility required for targeted cellular manipulation.

Purpose of the Study:

  • To develop and demonstrate the first endoscope-like integrated system for optical transfection.
  • To combine optical transfection with subcellular resolution imaging and microfluidic drug delivery.

Main Methods:

  • An imaging fiber bundle (∼300 μm diameter) with an integrated axicon lens array delivered femtosecond laser pulses for optical transfection.
  • A microcapillary-based microfluidic system was incorporated for localized therapeutic agent delivery.

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Last Updated: Jun 4, 2026

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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Published on: January 11, 2011

An &#8220;All-laser&#8221; Endothelial Transplant
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  • The system achieved subcellular resolution imaging alongside transfection capabilities.

    • Main Results:

    • Achieved a plasmid transfection efficiency of up to 72% in CHO-K1 cells.
    • Demonstrated the feasibility of an integrated, flexible endoscopic system for cellular manipulation.
    • Successfully combined optical transfection, imaging, and microfluidic delivery in a miniature device.

    Conclusions:

    • The developed endoscope-like system represents a significant advancement in targeted optical transfection.
    • This technology holds promise for applications in in vivo optical microsurgery and precise therapeutic agent delivery.