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Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
Published on: January 7, 2016
Human growth hormone (GH) immunoassay: standardization and clinical implications
Cinzia Carrozza1, Rosa Lapolla, Giulia Canu
1Institute of Biochemistry and Clinical Biochemistry, Catholic University School of Medicine, Rome, Italy. ccarrozza@rm.unicatt.it
Clinical Chemistry and Laboratory Medicine
|March 3, 2011
Summary
Comparing two growth hormone (GH) calibrators revealed good correlation but higher variability than expected. Adjusting GH therapy guidelines is recommended for better patient outcomes.
Area of Science:
- Endocrinology
- Clinical Chemistry
- Biotechnology
Background:
- Growth hormone (GH) assay comparability is hindered by standard preparation differences.
- The International Reference Preparation (IRP) IS 80/505 (human pituitary) and IRP IS 98/574 (recombinant 22K GH) are key calibrators.
- Evaluating the impact of these distinct calibrators on GH measurements is crucial.
Purpose of the Study:
- To assess the influence of two different calibrators (IS 80/505 and IS 98/574) on growth hormone (GH) results.
- To compare GH measurements obtained using the Siemens IMMULITE method with both calibrators.
Main Methods:
- GH levels in 97 pediatric serum samples from a GH stimulation test were analyzed.
- Siemens IMMULITE electro-chemiluminescence assay was employed, calibrated with both IS 80/505 and IS 98/574.
- Results were compared, and variations were analyzed across different GH concentration subgroups.
Main Results:
- A good correlation was observed between GH results obtained using the two calibrators.
- Higher percentage variations (var%) were noted than those reported by the manufacturer.
- Consistent mean var% was found across high and low GH concentration subgroups.
Conclusions:
- Standardization of GH assays is essential due to influencing factors like binding proteins and isoforms.
- Current Italian therapeutic GH values may require adjustment based on calibrator differences.
- Proposed adjustments to GH values will improve patient management for GH-related disorders.
