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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Updated: Jun 4, 2026

A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
09:46

A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy

Published on: January 17, 2018

Three-dimensional microscopic elemental analysis using an automated high-precision serial sectioning system.

Kazuhiro Fujisaki1, Hideo Yokota, Naomichi Furushiro

  • 1Division of Human Mechanical Systems and Design, Graduate School of Engineering, Hokkaido University, N13 W8, Kita-ku, Sapporo 060-8628, Japan.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|March 3, 2011
PubMed
Summary
This summary is machine-generated.

A new 3D elemental analysis system uses serial sectioning and X-ray fluorescence to map material inclusions. This automated system accurately reveals internal structures and elemental compositions for fracture analysis.

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Last Updated: Jun 4, 2026

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09:46

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Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
10:00

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles

Published on: July 5, 2016

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Microscopy

Background:

  • Inclusion analysis is crucial for understanding material fracture behavior.
  • Existing methods for observing internal material structures can be limited.

Purpose of the Study:

  • To develop an automated three-dimensional (3D) microscopic elemental analysis system.
  • To enable detailed observation of internal material structures and elemental composition.

Main Methods:

  • A serial sectioning technique was employed, integrating an X-ray fluorescence analyzer and a high-precision milling machine.
  • Automated control signals synchronized X-ray observation with precision positioning during the milling process.
  • Composite specimens with known structures were used for system validation.

Main Results:

  • The developed system successfully generated accurate 3D models of internal material structures.
  • The system clearly visualized the twisted wire structure within composite specimens.
  • Elemental composition and microscopic shape of inclusions were successfully mapped.

Conclusions:

  • The automated 3D elemental analysis system provides a powerful tool for material investigation.
  • This technique enhances the study of material fracture by detailing internal features.
  • The system facilitates automated, high-resolution analysis of material internal structures and elemental components.