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Related Experiment Video

Updated: Jun 4, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

[Screening drugs for regulating tissue factor gene expression].

Yan Yang¹, Fan-Zhi Yan, Jin-Song Yan

¹Department of Hematology, Dalian Medical University Second Hospital, Dalian 116027, Liaoning Province, China.

Zhongguo Shi Yan Xue Ye Xue Za Zhi

|March 3, 2011

Summary

This summary is machine-generated.

Researchers developed a stable cell line to screen drugs regulating tissue factor (TF) gene expression. Bortezomib (BTZ) activated TF transcription, while CDA-II suppressed it, identifying novel drug candidates.

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Last Updated: Jun 4, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

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Published on: November 22, 2024

Extracellular Vesicle Tissue Factor Activity Assay

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Published on: December 29, 2023

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

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Published on: February 13, 2013

Area of Science:

Molecular Biology
Gene Regulation
Drug Discovery

Context:

Tissue factor (TF) plays a critical role in hemostasis and thrombosis.
Understanding TF gene regulation is crucial for developing targeted therapies.
Existing methods for screening TF-regulating drugs are limited.

Purpose:

To establish a stable U937 cell line expressing luciferase under the control of the TF promoter.
To screen for novel drugs that modulate TF gene expression.
To identify the specific TF promoter region responsive to drug treatment.

Summary:

Stable U937 cell lines with TF promoter-luciferase constructs were created.
Bortezomib (BTZ) at 5 nmol/L was found to activate TF transcription.
CDA-II at 1 mg/ml was found to suppress TF transcription.
The critical regulatory region for BTZ and CDA-II activity was mapped to -201 to 0 bp of the TF promoter.

Impact:

Successful establishment of a novel cell line for TF research.
Identification of BTZ and CDA-II as potential modulators of TF expression.
Provides a foundation for further investigation into the molecular mechanisms of TF regulation and drug action.