Purification of tumor mitochondrial malic enzyme by specific ligand affinity chromatography
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Updated: Jul 20, 2026

11:32
Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
Published on: August 6, 2021
1Department of Pharmacology, University of Wisconsin Medical School, Madison 53706.
Protein Expression and Purification
|November 1, 1990
Abstract:
A two-step chromatographic procedure, based on a specific ligand-binding approach, for the purification of tumor NAD(P)(+)-dependent malic enzyme is described. The enzyme was purified to near homogeneity by extraction from mitochondria, negative cellulose phosphate chromatography, ammonium sulfate precipitation, and application of specific elution from a malate-agarose column. The rationale for the use of the affinity column is also described.

