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Published on: June 13, 2016
Protein profile of exosomes from trabecular meshwork cells
W D Stamer1, E A Hoffman, J M Luther
1Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, AZ 85711, United States. dstamer@eyes.arizona.edu
Journal of Proteomics
|March 3, 2011
Summary
Researchers analyzed the proteome of exosomes from human trabecular meshwork (TM) cells to understand their role in controlling intraocular pressure. They identified unique proteins, including myocilin, suggesting exosomes are involved in regulating eye pressure.
Area of Science:
- Ophthalmology
- Cell Biology
- Proteomics
Background:
- The trabecular meshwork (TM) is crucial for regulating intraocular pressure (IOP).
- Exosomes are small vesicles involved in intercellular communication, but their role in the TM is not fully understood.
Purpose of the Study:
- To characterize the proteome of exosomes derived from human TM cells.
- To investigate the potential role of TM cell-derived exosomes in IOP regulation.
Main Methods:
- Primary human TM cell cultures were established.
- Exosomes were purified from conditioned media and urine.
- Proteomic analysis was performed using 2D HPLC and MudPIT (Multi-Dimensional Protein Identification Technology) with MS/MS (tandem mass spectrometry).
- Protein profiles were compared to ExoCarta database and validated by Western blot.
Main Results:
- TM cell exosomes contained 108 of the 143 most represented exosome proteins in ExoCarta.
- Unique TM cell-specific proteins, including myocilin, emilin-1, and neuropilin-1, were identified.
- Exosome protein characteristics (flotation density, ionomycin response) were confirmed.
Conclusions:
- Human TM cell exosomes possess a characteristic exosome protein profile.
- TM exosomes contain unique proteins, notably myocilin, a protein linked to glaucoma.
- These findings suggest a role for TM exosomes in the physiological control of intraocular pressure.
