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Updated: Jun 4, 2026

Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper (Capsicum annuum L.)

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Published on: November 30, 2022

A single subunit MCM6 from pea promotes salinity stress tolerance without affecting yield.

Hung Quang Dang¹, Ngoc Quang Tran, Sarvajeet Singh Gill

¹International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.

Plant Molecular Biology

|March 3, 2011

Summary

Overexpressing the MCM6 gene in tobacco plants enhances salinity tolerance, allowing them to grow and produce seeds under salt stress without yield loss. This suggests DNA replication proteins can improve crop stress resistance.

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Published on: March 30, 2018

Area of Science:

Plant Molecular Biology
Genetics and Genomics
Biotechnology

Background:

The eukaryotic pre-replicative complex (Pre-RC), including Minichromosome Maintenance (MCM2-7) proteins, is crucial for DNA replication, ensuring genome stability.
MCM proteins' role in abiotic stress tolerance in plants remains largely unexplored, despite their importance in cell division and potential stress sensitivity.

Purpose of the Study:

To investigate the role of MCM6 in plant abiotic stress response.
To determine if MCM6 overexpression enhances stress tolerance in plants.

Main Methods:

Analyzing MCM6 transcript levels in pea plants under various stress conditions (salinity, cold, ABA, drought, heat).
Generating transgenic tobacco plants overexpressing MCM6 using the CaMV-35S promoter.
Evaluating the growth, yield, ion accumulation, chlorophyll content, and photosynthetic rates of transgenic and wild-type plants under salinity stress.

Main Results:

MCM6 transcript was upregulated in pea plants under high salinity and cold stress, but not ABA, drought, or heat.
MCM6-overexpressing tobacco plants (T1 generation) exhibited significant salinity tolerance, growing to maturity and producing viable seeds without yield penalty.
Transgenic plants showed reduced Na+ accumulation in seeds, maintained higher chlorophyll levels, net photosynthetic rates, dry matter accumulation, and yield under 200 mM NaCl compared to wild-type.

Conclusions:

Overexpression of the MCM6 gene confers significant salinity stress tolerance in tobacco plants without compromising yield.
MCM6 plays a direct role in plant salinity stress response.
The DNA replication machinery, specifically MCM6, presents a potential target for engineering stress tolerance in crop plants.