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Updated: Jun 4, 2026

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

Construction of small RNA cDNA libraries for high-throughput sequencing.

Cheng Lu¹, Vikas Shedge

¹Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA. lu@dbi.udel.edu

Methods in Molecular Biology (Clifton, N.J.)

|March 3, 2011

Summary

Small RNAs regulate gene expression for growth and development. This study details a method for constructing small RNA cDNA libraries for high-throughput sequencing analysis.

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Last Updated: Jun 4, 2026

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

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Published on: October 27, 2011

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Small RNAs (smRNAs) are crucial regulators of gene expression.
smRNAs influence diverse biological processes, including growth and development.
High-throughput sequencing enables comprehensive smRNA profiling.

Purpose of the Study:

To describe a laboratory method for constructing smRNA cDNA libraries.
To facilitate high-throughput sequencing of smRNA populations.

Main Methods:

Construction of small RNA (smRNA) complementary DNA (cDNA) libraries.
Application of high-throughput sequencing technologies.

Main Results:

A detailed protocol for smRNA cDNA library preparation was established.
The method allows for comprehensive analysis of smRNA populations.

Conclusions:

The described method provides a robust approach for smRNA research.
This technique supports the investigation of smRNA roles in various biological contexts.