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Updated: Jun 4, 2026

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
Published on: April 8, 2017
Generation of a large catalog of unique transcripts for whole-genome expression analysis in nonmodel species
Diana Bellin1, Alberto Ferrarini, Massimo Delledonne
1Department of Biotechnology, University of Verona, Verona, Italy.
Abstract:
Next-generation sequencing technologies have allowed new tools to be developed for transcriptome characterization. We describe here the methods for the preparation of a library from pooled RNAs for 454 sequencing of 3'-cDNA fragments. We also describe how the read sequences obtained can be used to generate, through de novo reads assembly, a large catalog of unique transcripts in organisms for which a comprehensive collection of transcripts or the complete genome sequence is not available. Finally, this catalog can be used efficiently to design oligonucleotide probes for setting up a comprehensive microarray for global analysis of gene expression.
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