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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
Published on: March 24, 2012
Redox-regulatory mechanisms induced by oxidative stress in Brassica juncea roots monitored by 2-DE proteomics
Sophie Alvarez1, Ashley Galant, Joseph M Jez
1Donald Danforth Plant Science Center, St. Louis, MO 63132, USA.
Abstract:
ROS, including hydrogen peroxide (H(2)O(2)), can serve as cellular signaling molecules following oxidative stress. Analysis of the redox state of proteins in Brassica juncea roots by 2-DE proteomics following treatment with either exogenous H(2)O(2) or buthionine sulfoximine, which depletes glutathione to cause accumulation of endogenous H(2)O(2), led to the identification of different sets of proteins. These data suggest that exogenous and endogenous oxidative stresses trigger specialized responses.
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