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Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
Published on: March 29, 2018
Segmentation and surface reconstruction of the detailed ear structures, identified in sectioned images
Hae Gwon Jang1, Min Suk Chung, Dong Sun Shin
1Multimedia and Computer Architecture Lab, Graduate School of Information and Communication, Ajou University, Worldcup-ro 206, Suwon, Republic of Korea.
Abstract:
The structure of the ear, which intervenes between gross anatomy and histology in size, can be best understood by means of three-dimensional (3D) surface models on a computer. Furthermore, surface models are the source of interactive simulation for clinical trials, such as tympanoplasty. The objective of this research was to elaborate the surface models of detailed ear structures, which contribute to learning anatomy or the practice of otology. We produced sectioned images of a cadaver head (pixel size, 0.1 mm; 48-bit color). In the sectioned images, the external, middle, and internal ear structures and other related components were delineated on Photoshop to acquire segmented images at 0.5-mm intervals. Segmented images of each structure were stacked, and the surface was reconstructed to generate a 3D-surface model on commercial software. Thirty surface models showed fine ear topographic anatomy (e.g., semicircular ducts), as expected. Herein, we present the corresponding sectioned images, segmented images, and surface models of ear structures that will be released together. It is hoped that these image data will stimulate the development of medical simulations. The efficient technique of segmentation and surface reconstruction enables the manufacture of surface models from other serial images (e.g., CTs and MRIs).

