[Adhesion of osteoblasts on the novel titanium alloy with bioactivity and wear coating]

The purpose of this study was to evaluate the rugosity with both of the bioactivity and the wear coating of a new type titanium implant material Ti-Zr-Sn-Mo-Nb(TZS), and to investigate the adhesion of osteoblasts on different coating surface.

Both surface topography of bioactivity and wear coating were examined with scanning electron microscope (SEM). The average roughness of two kinds coating were measured by portable roughometer. We examined the dynamic behavior of early adhesion of the osteoblasts, which were cultured on different coating surface of titanium alloy. As a marker of cell adhesions, cytoskeleton proteins were tagged by F-actin agent. Additionally, the number of adhesion cells was investigated by biological assay MTT. These results were compared with those founds in the control.

Bioactivity-coating with pore structure and wear-coating with irregular pitting corrosion were observed. Roughometer examination revealed that the roughness of different disc was increased. The control bioactivity-coating surface was the most rough, followed by wear-coating and then the control. Bioactivity coating had best cell biocompatibility, in that the highest cell adhesion ratio was detected. It was very likely that bioactivity coating possessed of biomimetic character and roughness surface. Wear coating had similar biocompatible in comparison with bioactivity coating. The cytoskeleton protein of cell formed a fibrillar structure on bioactivity and wear coating. Cytoskeleton tensin was heterogeneously distributed on variety of surface depending on different surface topography.

The new titanium alloy shows rough surface after surface treatment, and then cell adhesion and morphology, cytoskeleton protein present wea on bioactivity-coating and wear-coating.