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[The effect of ginkgolic acids on multidrug resistance in oral squamous cell carcinoma]

Hui Wang1, Chen-Chen Zhou, Yun Feng

  • 1State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.

Hua Xi Kou Qiang Yi Xue Za Zhi = Huaxi Kouqiang Yixue Zazhi = West China Journal of Stomatology
|March 4, 2011
PubMed
Abstract

Insights

Ginkgolic acids partially reverse multidrug resistance (MDR) in carboplatin and pingyangmycin-resistant cancer cells. This study demonstrates ginkgolic acids

Area of Science:

  • Oncology
  • Pharmacology
  • Cell Biology

Background:

  • Multidrug resistance (MDR) is a major challenge in cancer chemotherapy, limiting the efficacy of drugs like carboplatin (CBP) and pingyangmycin (PYM).
  • Drug-resistant cell lines, such as Tca8113/CBP and Tca8113/PYM, are crucial for studying MDR mechanisms and evaluating potential therapeutic strategies.

Purpose of the Study:

  • To investigate the influence of ginkgolic acids on multidrug resistance (MDR) in established CBP-resistant (Tca8113/CBP) and PYM-resistant (Tca8113/PYM) cell lines.
  • To determine the efficacy of combining ginkgolic acids with chemotherapy drugs in overcoming drug resistance.

Main Methods:

  • Establishment of in vitro CBP-resistant (Tca8113/CBP) and PYM-resistant (Tca8113/PYM) cell lines.
  • Immunohistochemistry to detect P-glycoprotein (P-gp) expression.
  • MTT assays to determine cell proliferation, resistance indices, and non-toxic concentrations of ginkgolic acids.

Main Results:

  • Drug-resistant cells exhibited significantly higher P-gp expression compared to parental cells.
  • A non-toxic concentration of ginkgolic acids was determined to be 10 microg/mL.
  • Ginkgolic acids partially reversed resistance, with reversal folds of 2.94 for Tca8113/CBP and 2.43 for Tca8113/PYM.
  • Combined treatment with ginkgolic acids and chemotherapy drugs reduced resistance indices to 2.18 for Tca8113/CBP and 4.43 for Tca8113/PYM.

Conclusions:

  • Successfully established CBP- and PYM-resistant cell lines (Tca8113/CBP and Tca8113/PYM).
  • Ginkgolic acids, at non-toxic concentrations, can partially reverse drug resistance in these cell lines.
  • Combining ginkgolic acids with chemotherapy drugs demonstrates a reduced effect on cancer cell proliferation and a decrease in MDR levels.

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