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[Comparison of six molecular methods for differentiation of Lactococcus lactis subsp. lactis and cremoris]
Jiachao Zhang1, Fang Wang, Haiyan Xu
1Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China. zhjch321123@sohu.com
Objective:
To compare six molecular methods for differentiation of Lactococcus lactis subsp. lactis and cremoris.
Methods:
Six molecular methods, such as 16S rRNA gene analysis, 16S-23S rRNA Intergenic spacer region polymorphism, Denaturing Gradient Gel Electrophoresis (DGGE), Random Amplified Polymorphic DNA (RAPD), Repetitive Extragenic Palindromic-PCR and Restricted Fragment Length Polymorphisms were used to differentiate the reference strains.
Results:
Except for 16S rRNA gene analysis and 16S-23S rRNA Intergenic spacer region polymorphism, the other protocols were competent.
Conclusion:
The methods of DGGE and RAPD were more appropriate for differentiation of Lactococcus lactis subsp. lactis and cremoris.
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