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Naturally activated and resting T cells differ in their activation requirements for growth and secretory activities
A F Nóbrega1, G A DosReis, R Fucs
1Department of Immunology, Federal University of Rio de Janeiro, Brazil.
"Naturally activated" (NA) and "small resting" (SR) T lymphocytes were stimulated with anti-CD3 or anti-thy 1 monoclonal antibodies (mAbs). Both proliferation and secreted IL-2, IL-3/GM-CSF activities were found in NA T cell, but not in SR T cell cultures. SR T cells could be fully activated by anti-CD3 only if PMA or IL-2 was added to the cultures. NA T cell proliferation induced with anti-CD3 was blocked with anti-IL-2 or anti-IL-2R mAbs. The combination of anti-CD3 and rec IL-4 was not effective in promoting SR T cell proliferation. IL-4 plays a minor role in NA T cell activation with anti-CD3, as assayed with neutralizing anti IL-4 mAbs. No differences in the proliferative and secretory activities were found when NA or SR T cells were stimulated with Con A. Both NA and SR T cells responded when stimulated with the calcium ionophone A23187 plus PMA. Only NA T cells responded to A23187 alone. The mechanisms and the possible physiologic relevance of this differential responsiveness behavior are discussed.
"Naturally activated" (NA) and "small resting" (SR) T lymphocytes were stimulated with anti-CD3 or anti-thy 1 monoclonal antibodies (mAbs). Both proliferation and secreted IL-2, IL-3/GM-CSF activities were found in NA T cell, but not in SR T cell cultures. SR T cells could be fully activated by anti-CD3 only if PMA or IL-2 was added to the cultures. NA T cell proliferation induced with anti-CD3 was blocked with anti-IL-2 or anti-IL-2R mAbs. The combination of anti-CD3 and rec IL-4 was not effective in promoting SR T cell proliferation. IL-4 plays a minor role in NA T cell activation with anti-CD3, as assayed with neutralizing anti IL-4 mAbs. No differences in the proliferative and secretory activities were found when NA or SR T cells were stimulated with Con A. Both NA and SR T cells responded when stimulated with the calcium ionophone A23187 plus PMA. Only NA T cells responded to A23187 alone. The mechanisms and the possible physiologic relevance of this differential responsiveness behavior are discussed.