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Core promoter recognition complex changes accompany liver development.

Joseph A D'Alessio¹, Raymond Ng, Holger Willenbring

¹Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

Proceedings of the National Academy of Sciences of the United States of America

|March 4, 2011

Summary

This summary is machine-generated.

During mouse liver development, key transcriptional regulators, transcription factor IID (TFIID) and cofactor required for Sp1 activation/Mediator, are depleted. This suggests a shift in gene regulation during cellular differentiation.

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Area of Science:

Molecular Biology
Developmental Biology
Gene Regulation

Background:

The basal transcriptional apparatus was historically considered ubiquitous and invariant.
Cellular differentiation involves complex changes in gene expression.
Core promoter recognition and coactivator complex switching are critical for regulating transcription during development.

Purpose of the Study:

To investigate the role of TFIID and Mediator complexes in mouse liver development.
To understand how core promoter recognition and coactivator complex switching influence cellular differentiation.

Main Methods:

Examined changes in transcription factor IID (TFIID) and cofactor required for Sp1 activation/Mediator during mouse liver development.
Analyzed RNA and protein levels of these complexes.

Main Results:

Fetal liver progenitor differentiation into adult hepatocytes involves significant depletion of TFIID and Mediator complexes at both RNA and protein levels.
This depletion is associated with the silencing of transcription factor promoters and protein degradation.

Conclusions:

The canonical TFIID and Mediator complexes are dynamically regulated during liver development.
Mechanisms for TFIID downregulation and the potential involvement of novel core promoter recognition complexes in adult hepatocytes warrant further investigation.