Related Experiment Video
Updated: Jun 4, 2026
An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
Published on: September 19, 2018
Focusing transgene expression in Drosophila by coupling Gal4 with a novel split-LexA expression system
Chun-Yuan Ting1, Stephanie Gu, Sudha Guttikonda
1Eunice Kennedy Shriver National Institute of Child Healthand Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
None:
Here we report the development of a ternary version of the LexA::VP16/LexAop system in which the DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA system can be concatenated with the Gal4/upstream activating sequence system to refine the expression patterns of existing Gal4 lines with minimal genetic manipulations.
