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Updated: Jun 4, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
Invasive potential of biofilm-forming Staphylococci bovine subclinical mastitis isolates
Manuela Oliveira1, Ricardo Bexiga, Sandro Filipe Nunes
1CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, 1300-477 Lisbon, Portugal. moliveira@fmv.utl.pt
Abstract:
Staphylococcus (S.) aureus is a common infectious agent of bovine chronic mastitis, a disease that is difficult to eradicate. The abilities of staphylococci to be internalized and form a biofilm can contribute to host immunological defence evasion that subsequently impairs antimicrobial therapy. The invasive capability of six S. aureus field isolates with different biofilmforming profiles was compared in vitro using a bovine mammary epithelial cell line. This was further confirmed in primary cell cultures using fluorescent rRNA probes against S. aureus. The results suggest that S. aureus invasion levels are not related to biofilm formation.
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