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Related Experiment Video

Updated: Jun 4, 2026

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

MicroRNA isolation from formalin-fixed, paraffin-embedded tissues.

Aihua Liu1, Xiaowei Xu

  • 1University of Pennsylvania School of Medicine, Philadelphia, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 4, 2011
PubMed
Summary

Formalin-fixed, paraffin-embedded (FFPE) tissues are suitable for microRNA (miRNA) expression analysis. miRNA profiles from FFPE samples closely match those from fresh-frozen tissues, validating FFPE utility.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are crucial noncoding RNAs regulating gene expression.
  • Aberrant miRNA expression is linked to various human diseases, including cancer.
  • Formalin-fixed, paraffin-embedded (FFPE) tissues are abundant but RNA quality is a concern.

Purpose of the Study:

  • To assess the suitability of FFPE tissues for miRNA expression profiling.
  • To compare miRNA expression profiles between FFPE and fresh-frozen tissues.
  • To validate FFPE tissues as a resource for miRNA research.

Main Methods:

  • RNA extraction from matched FFPE and fresh-frozen clinical tissue samples.
  • Microarray analysis to compare miRNA expression profiles.

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MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
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MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

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Last Updated: Jun 4, 2026

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

  • Comparative analysis of miRNA expression data.

    • Main Results:

    • miRNA expression profiles from FFPE tissues closely resemble those from fresh-frozen tissues.
    • High correlation observed between miRNA expression in FFPE and fresh samples.
    • FFPE tissues yield reliable miRNA expression data.

    Conclusions:

    • FFPE tissues are a valuable and appropriate resource for miRNA expression analyses.
    • The study validates the use of archived FFPE samples for miRNA research.
    • Findings support the utility of FFPE tissues in disease-related miRNA studies.