Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Programming CO and syngas production under large current density via lanthanide family-guided doped silver aerogels in electrochemical CO<sub>2</sub> reduction.

Journal of colloid and interface science·2026
Same author

Methodological approaches for estimating radiosensitivity in <i>Quillaja saponaria</i>: comparison of LD<sub>50</sub> models and a PCA-based integrative index.

International journal of radiation biology·2026
Same author

Distinct Domains Contribute to the Subcellular Localization of Human cGAS in Yeast.

Biomolecules·2026
Same author

Hidradenitis suppurativa in patients with inflammatory bowel disease: a national multicenter study from the GETECCU-ENEIDA Registry.

Therapeutic advances in gastroenterology·2026
Same author

First prospective, single-arm, multicenter study to evaluate the safety and efficacy of the overall thrombectomy system for stroke: iNedit, iNdeep and iNtercept in patients with acute ischemic stroke (SEMTiC-01 study).

AJNR. American journal of neuroradiology·2026
Same author

Integration of human ERKs and DUSPs into the yeast cell wall integrity pathway.

Scientific reports·2025

Related Experiment Video

Updated: Jun 4, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Reverse protein arrays applied to host-pathogen interaction studies.

Víctor J Cid1, Ekkehard Kauffmann, María Molina

  • 1Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|March 4, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces Reverse Protein Arrays (RPAs) to investigate how pathogens like Salmonella typhimurium manipulate host cell signaling pathways during infection. This novel approach aids in understanding infectious diseases for potential therapeutic interventions.

More Related Videos

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Published on: October 20, 2020

Related Experiment Videos

Last Updated: Jun 4, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Published on: October 20, 2020

Area of Science:

  • Microbiology
  • Cell Biology
  • Proteomics

Background:

  • Pathogenic microorganisms reprogram host cell signaling for replication and immune evasion.
  • Understanding host cell subversion by intracellular pathogens is crucial for developing interventions.
  • Reverse Protein Arrays (RPAs) are established in oncology but underexplored in infectious disease research.

Purpose of the Study:

  • To explore the application of Reverse Protein Arrays (RPAs) in studying host-pathogen interactions.
  • To investigate molecular mechanisms of host cell manipulation by intracellular pathogens.
  • To adapt proteomic approaches for infectious disease research.

Main Methods:

  • Utilizing classic in vitro infection techniques combined with proteomic analysis.
  • Employing Reverse Protein Arrays (RPAs) to analyze host cell signaling.
  • Monitoring global phosphorylation changes using antibodies specific for phosphorylated epitopes.

Main Results:

  • Demonstrated the potential of RPA technology in infectious disease research.
  • Developed a microarray using cell culture lysates infected with Salmonella typhimurium.
  • Established protocols for applying RPA analysis in this context.

Conclusions:

  • RPAs offer a powerful tool for dissecting host cell pathway manipulation by pathogens.
  • This proteomic approach provides insights into host-pathogen molecular interactions.
  • The described protocols facilitate the study of infectious diseases using RPAs.