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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Updated: Jun 4, 2026

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

DNA extraction from paraffin-embedded tissues.

H Fan¹, M L Gulley

¹Department of Pathology, University of Texas Health Science Center, Sun Antonio, TX.

Methods in Molecular Medicine

|March 4, 2011

Summary

This summary is machine-generated.

This study details a protocol for extracting DNA from formalin-fixed, paraffin-embedded tissues. This method prepares DNA from archival tissue samples for molecular analysis, enabling further genetic research.

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Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

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Last Updated: Jun 4, 2026

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

Area of Science:

Histopathology
Molecular Biology
Biochemistry

Background:

Formalin-fixed, paraffin-embedded (FFPE) tissues are standard for long-term preservation in histopathology.
DNA extraction from FFPE tissues is crucial for molecular analyses like PCR and sequencing.
Existing protocols require optimization for efficient DNA recovery.

Purpose of the Study:

To present a refined protocol for DNA extraction from FFPE tissues.
To ensure DNA quality and yield suitable for amplification-based molecular techniques.

Main Methods:

Tissue sections from FFPE blocks were processed.
Deparaffinization using xylene followed by ethanol washes.
Proteinase K treatment to facilitate DNA release for amplification.

Main Results:

The protocol effectively isolates DNA from FFPE samples.
Prepared DNA is suitable for downstream molecular amplification.
The method is based on established procedures, ensuring reliability.

Conclusions:

This protocol provides a reliable method for DNA preparation from FFPE tissues.
The extracted DNA is viable for various molecular analyses.
This technique supports the utility of archival tissue samples in research.