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A Nested Reverse Transcription-Polymerase Chain Reaction Assay to Detect BCR/abl

L M Wasserman1

  • 1Division of Medical Genetics, Department of Genetics, Department of Medicine, University of California, San Diego, La Jolla, CA.

Methods in Molecular Medicine
|March 4, 2011
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...