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Updated: Jun 4, 2026

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Measuring receptor-mediated cell adhesion under flow : cell-free systems.

D A Hammer¹, D K Brunk

¹Department of Chemical Engineering, University of Pennsylvania, Philadelphia, PA.

Methods in Molecular Medicine

|March 4, 2011

Summary

Leukocyte adhesion to vascular endothelium under flow is crucial for immune cell trafficking. This process involves rolling, firm attachment, and migration, mediated by specific adhesion receptors and counterreceptors.

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Area of Science:

Immunology
Cell Biology
Physiology

Background:

Leukocytes must adhere to vascular endothelium to exit blood vessels and perform immune functions.
This trafficking is essential for neutrophils in inflammation and lymphocytes in lymphoid tissue.
Leukocyte egress involves a series of adhesion-dependent steps mediated by specific receptors.

Purpose of the Study:

To elucidate the fundamental mechanisms of leukocyte adhesion and trafficking across the vascular endothelium.
To highlight the critical role of leukocyte adhesion receptors and their counterreceptors in this process.

Main Methods:

Investigated leukocyte-endothelial cell interactions under physiological flow conditions.
Focused on the molecular interactions governing transient adhesion (rolling) and firm attachment.

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Last Updated: Jun 4, 2026

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

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Published on: November 2, 2011

Quantitation of Endothelial Cell Adhesiveness In Vitro

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Published on: June 18, 2015

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

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Main Results:

Leukocyte adhesion and trafficking are complex, multi-step processes.
Transient adhesion, or rolling, is a necessary precursor to firm attachment and subsequent migration.
Specific leukocyte adhesion molecules and endothelial counterreceptors orchestrate these interactions.

Conclusions:

Understanding leukocyte adhesion mechanisms is key to modulating immune responses.
Targeting these adhesion events could offer therapeutic strategies for inflammatory and immune diseases.