Isolation and characterization of a novel pyrene-degrading Bacillus vallismortis strain JY3A

The PAHs-degrading bacterium strain JY3A was newly isolated from the polluted soil in the Jinan Oil Refinery Factory, Shandong Province of China. The isolate was identified as Bacillus vallismortis with respect to its 16S rDNA sequence, DNA-DNA relatedness and fatty acid profiles, as well as various physiological characteristics. The strain was Gram-positive, motile, endospore forming, aerobic, oxidase and catalase-positive. The cells were 0.8-1.0μm wide and 2.0-2.5μm long, single or in pairs and sometimes in chains. Bacillus vallismortis strain JY3A could utilize naphthalene, phenanthrene, anthracene, pyrene, fluorene, benzene, toluene, phenol, methanol, ethanol, Tween 80, cyclohexane or catechol as sole carbon source. The strain alone removed 90.5% of pyrene at an initial concentration of 150ppm in 15days in the presence of 0.5% (w/w) Tween 80. However, in co-culture with Phanerochaete chrysosporium, JY3A reduced the concentration of pyrene by nearly 55.4% after 7days of incubation.