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Updated: Jun 3, 2026

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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Transcriptional repression in the Notch pathway: thermodynamic characterization of CSL-MINT (Msx2-interacting nuclear

Bradley D VanderWielen1, Zhenyu Yuan, David R Friedmann

  • 1Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267, USA.

The Journal of Biological Chemistry
|March 5, 2011
PubMed

Insights

The Notch pathway

Area of Science:

  • Molecular biology
  • Cell signaling
  • Developmental biology

Background:

  • The Notch pathway regulates cell fate decisions via gene expression changes.
  • CSL protein acts as a transcriptional regulator, functioning as either a repressor or activator.
  • The structure and function of CSL-corepressor complexes are not well understood.

Purpose of the Study:

  • To quantitatively analyze the thermodynamic binding of CSL and MINT.
  • To define the domains of MINT and CSL involved in complex formation.
  • To investigate the functional impact of MINT-CSL interaction on Notch signaling.

Main Methods:

  • Isothermal titration calorimetry (ITC) for binding analysis.
  • Cellular assays to assess Notch signaling inhibition.
  • Domain mapping of MINT and CSL interaction.

Main Results:

  • MINT forms a high-affinity complex with CSL.
  • Specific domains of MINT and CSL were identified as necessary and sufficient for binding.
  • The MINT-CSL interaction region inhibits Notch signaling in cultured cells.

Conclusions:

  • MINT is a high-affinity binding partner of CSL.
  • The MINT-CSL interaction provides functional insights into Notch signaling regulation.
  • This study clarifies how CSL transitions between repressor and activator roles.

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