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Updated: Jun 3, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

Multilocus sequence typing of Mycoplasma agalactiae.

L McAuliffe¹, F Gosney¹, M Hlusek¹

¹Veterinary Laboratories Agency (Weybridge), Woodham Lane, Addlestone, Surrey KT15 3NB, UK.

Journal of Medical Microbiology

|March 5, 2011

Summary

A new multilocus sequence typing (MLST) scheme for Mycoplasma agalactiae effectively differentiates strains, aiding in understanding contagious agalactia epidemiology in sheep and goats.

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Last Updated: Jun 3, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

Area of Science:

Veterinary Microbiology
Molecular Epidemiology
Bacterial Genomics

Background:

Contagious agalactia, caused by Mycoplasma agalactiae, significantly impacts sheep and goat health and economies.
Accurate strain differentiation is crucial for epidemiological surveillance and control of this disease.

Purpose of the Study:

To develop and validate a multilocus sequence typing (MLST) scheme for Mycoplasma agalactiae.
To assess the discriminatory power of the MLST scheme for epidemiological studies.

Main Methods:

Developed an MLST scheme targeting five housekeeping genes (gltX, metS, gyrB, tufA, dnaA).
Analyzed 3468 bp of sequence data per strain.
Applied the scheme to 53 Mycoplasma agalactiae isolates.

Main Results:

The MLST scheme successfully subdivided 53 strains into 17 distinct sequence types, correlating largely with geographical origin.
Identified novel genetic diversity in Spanish isolates and enabled typing of Mongolian isolates.
Mutation was the primary driver of genetic diversity, with limited evidence of recombination.

Conclusions:

The developed MLST scheme is a robust and highly discriminatory tool for Mycoplasma agalactiae molecular epidemiology.
Facilitates interlaboratory data comparison and enhances surveillance of contagious agalactia.
A web-accessible database (http://pubmlst.org/magalactiae/) is available for the MLST scheme.