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Related Experiment Video

Updated: Jun 3, 2026

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns
04:24

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

Published on: February 13, 2011

Studying cell rolling trajectories on asymmetric receptor patterns.

Chia-Hua Lee1, Suman Bose, Krystyn J Van Vliet

  • 1Department of Materials Science and Engineering, MIT-Massachusetts Institute of Technology, USA.

Journal of Visualized Experiments : Jove
|March 5, 2011
PubMed
Summary

Cells rolling on patterned receptors show lateral displacement, enabling new label-free cell separation devices. This study quantifies cell rolling trajectories to optimize device design for cell analysis and separation.

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Area of Science:

  • Biophysics
  • Cellular Mechanics
  • Microfluidics

Background:

  • Cell rolling adhesion on patterned receptors can cause lateral displacement, offering potential for label-free cell separation and analysis.
  • Understanding cell rolling trajectories is crucial for designing effective microfluidic devices that utilize this phenomenon.

Purpose of the Study:

  • To develop and demonstrate a protocol for studying cell rolling trajectories on asymmetric receptor patterns.
  • To quantify cell rolling velocities and edge tracking lengths on engineered receptor patterns.

Main Methods:

  • Fabrication of μm-scale P-selectin receptor patterns using microcontact printing on gold-coated slides.
  • Flowing HL60 cells (expressing PSGL-1) over patterned substrates in a flow chamber and visualizing their rolling behavior.

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Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
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Last Updated: Jun 3, 2026

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns
04:24

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

Published on: February 13, 2011

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay
08:24

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
11:04

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Published on: October 16, 2013

  • Utilizing particle-tracking software to analyze cell rolling trajectories, velocities, and edge tracking lengths on individual receptor patterns.

    • Main Results:

    • Cells exhibited rolling adhesion and lateral deflection along inclined receptor pattern edges.
    • Quantified distributions of cell rolling velocities and edge tracking lengths for different pattern designs.
    • Demonstrated a protocol for relating cell rolling behavior to engineering parameters like pattern angle and shear stress.

    Conclusions:

    • The developed protocol enables quantification of cell rolling trajectories on receptor patterns.
    • This data is essential for the engineering of substrates and the design of microfluidic devices for label-free cell separation and analysis.
    • The findings support the development of advanced cell analysis and separation technologies.