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Related Experiment Video

Updated: Jun 3, 2026

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

HMGN5: a potential oncogene in gliomas.

Jintao Qu¹, Runmin Yan, Juxiang Chen

¹Department of Neurosurgery, Shanghai Institute of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai, 200433, China.

Journal of Neuro-Oncology

|March 5, 2011

Summary

High-mobility group nucleosome binding domain 5 (HMGN5) promotes glioma cell proliferation and tumorigenesis. Reducing HMGN5 expression inhibits glioma cell growth, suggesting it is a potential therapeutic target for brain tumors.

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Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

Area of Science:

Oncology
Molecular Biology
Neuroscience

Background:

Gliomas are common primary brain tumors and a leading cause of cancer death.
High-mobility group nucleosome binding domain 5 (HMGN5) is implicated as a potential oncogene in various cancers.

Purpose of the Study:

To investigate the role of HMGN5 in human glioma cell proliferation.
To determine if HMGN5 is essential for glioma tumorigenesis in vitro.

Main Methods:

Lentivirus-mediated RNA interference (RNAi) was used to decrease HMGN5 expression in U251 and U87 glioma cells.
Cell cycle progression, proliferation, apoptosis, and soft agar colony formation were analyzed.

Main Results:

Reduced HMGN5 expression led to G1 phase cell cycle arrest and delayed proliferation in U251 and U87 cells.
HMGN5 knockdown induced apoptosis and inhibited clonogenic growth in U251 cells.
HMGN5 was significantly overexpressed in both high-grade and low-grade glioma tissues compared to normal brain tissue.

Conclusions:

HMGN5 is crucial for glioma cell proliferation and tumorigenesis in vitro.
HMGN5 overexpression in glioma tissues suggests its involvement in glioma development.
HMGN5 represents a potential therapeutic target for glioma treatment.