Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Anticancer Metal Complexes: Synthesis and Cytotoxicity Evaluation by the MTT Assay

Anticancer Metal Complexes: Synthesis and Cytotoxicity Evaluation by the MTT Assay

Published on: November 10, 2013

Cell sensitivity assays : the MTT assay.

¹CRC Department of Medical Oncology, Beatson Laboratories, University of Glasgow, Glasgow, UK.

Methods in Molecular Medicine

|March 5, 2011

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluating the potential of polymer nanoparticles for oral delivery of paclitaxel in drug-resistant cancer.

Cancer nanotechnology·2015

Same author

Candidate DNA methylation drivers of acquired cisplatin resistance in ovarian cancer identified by methylome and expression profiling.

Oncogene·2012

Same author

Cell sensitivity assays : clonogenic assay.

Methods in molecular medicine·2011

Same author

Pharmacokinetic and pharmacodynamic properties of an oral formulation of the histone deacetylase inhibitor Belinostat (PXD101).

Cancer chemotherapy and pharmacology·2010

Same author

Combined inhibition of DNA methylation and histone acetylation enhances gene re-expression and drug sensitivity in vivo.

British journal of cancer·2009

Same author

CpG-island methylation and epigenetic control of resistance to chemotherapy.

Biochemical Society transactions·2004

Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015

Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014

Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014

Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014

Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014

Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014

See all related articles

A new, rapid cytotoxicity assay offers advantages over the traditional clonogenic assay for evaluating cell line sensitivity to cytotoxic agents and drug combinations. This method is quicker and more efficient for various cell types.

Area of Science:

Cell Biology
Pharmacology
Toxicology

Background:

The clonogenic assay, while established, has limitations for certain cell lines, including adherent and non-adherent types.
Its slow and time-consuming nature can hinder high-throughput screening and comparative analyses.
Existing cytotoxicity assessment methods may not be universally applicable or efficient.

Purpose of the Study:

To introduce and describe an alternative cytotoxicity assay.
To highlight the advantages of this new assay over the clonogenic assay.
To provide a method suitable for a broader range of cell lines and experimental designs.

Main Methods:

Description of a novel, rapid cytotoxicity assay.
Comparison of the new assay's performance and characteristics against the clonogenic assay.

More Related Videos

Screening Ion Channels in Cancer Cells

Screening Ion Channels in Cancer Cells

Published on: June 16, 2023

Related Experiment Videos

Last Updated: Jun 3, 2026

Anticancer Metal Complexes: Synthesis and Cytotoxicity Evaluation by the MTT Assay

Anticancer Metal Complexes: Synthesis and Cytotoxicity Evaluation by the MTT Assay

Published on: November 10, 2013

Screening Ion Channels in Cancer Cells

Screening Ion Channels in Cancer Cells

Published on: June 16, 2023

Focus on ease of use, speed, and batch processing capabilities.

Main Results:

The alternative assay is quick, easy to perform, and suitable for large batch processing.
It overcomes the limitations of the clonogenic assay regarding cell line applicability (adherent and non-adherent).
The assay facilitates efficient comparisons between cell lines, cytotoxic agents, and drug combinations.

Conclusions:

The described cytotoxicity assay presents a valuable, efficient alternative to the clonogenic assay.
Its speed and versatility make it ideal for drug discovery and comparative toxicology studies.
Results from this assay should ideally be corroborated with other methods for comprehensive validation.