Culturing Primitive Hemopoietic Cells : Long-Term Mouse Marrow Cultures and the Establishment of Factor-Dependent (FDCP-Mix) Hemopoietic Cell Lines
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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
Published on: January 6, 2009
1Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2011
Abstract:
The maintenance of normal primitive hemopoietic cell types in culture for long periods can be achieved either by culture of hemopoietic cells in association with bone marrow-derived stromal cells, as in long-term cultures, or by establishing factor-dependent primitive hemopoietic cell lines (FDCP-Mix cell lines). The long-term culture technique and a reproducible method for establishing FDCP-Mix cell lines will be described here (1,2).
