Production of human and murine eosinophils in vitro and assay for eosinophil differentiation factors

Bone marrow cells from a number of animal species have been used extensively in liquid and semisolid cultures to study hemopoiesis and to produce functional mature cells and factor-dependent cell lines (for review see ref. 1). Neutrophils and macrophages are produced without added growth factors from murine long-term bone marrow cultures (2), while lymphoid cells (3,4) and megakaryocytes (reviewed in ref. 5) can be induced under certain conditions.