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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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Related Experiment Video

Updated: Jun 3, 2026

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
17:38

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Published on: April 27, 2012

In Situ Hybridization with Radiolabeled cRNA Probes, Using Tissue Sections and Smears.

C Adams1, Q A Hamid, J M Polak

  • 1Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2011
PubMed
Summary

In situ hybridization allows detailed study of gene expression and functional morphology at the cellular level. This molecular biology technique overcomes limitations of immunocytochemistry for visualizing intracellular processes like protein synthesis.

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Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
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Last Updated: Jun 3, 2026

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
17:38

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Published on: April 27, 2012

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Immunocytochemistry and biochemical methods enable rapid identification of tissue components and cellular events like secretion.
  • These techniques, however, cannot monitor intracellular processes such as protein synthesis.
  • A need exists for methods providing detailed functional morphology and gene expression data at the cellular level.

Purpose of the Study:

  • To introduce and explain the principles of in situ hybridization.
  • To highlight its advantages over existing techniques for studying cellular processes.

Main Methods:

  • In situ hybridization utilizes labeled complementary probes (DNA or RNA) to detect specific nucleotide sequences (DNA or RNA).
  • This procedure is performed on tissue preparations.
  • Combines molecular biology with tissue analysis.

Main Results:

  • In situ hybridization provides detailed information on gene expression within tissues.
  • It allows visualization of specific nucleotide sequences at the cellular level.
  • Enables a better understanding of functional morphology and intracellular events.

Conclusions:

  • In situ hybridization is a powerful tool for investigating gene expression and functional morphology.
  • It overcomes the limitations of immunocytochemistry in studying protein synthesis and other intracellular events.
  • Advances in molecular biology have led to significant improvements in cellular and tissue analysis.