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Updated: Jun 3, 2026
10:45
iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
Published on: April 30, 2011
RNA extraction by the proteinase k method
1Inveresk Research International Limited, Musselburgh, Scotland.
Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2011
Abstract:
This method of RNA extraction relies on a relatively gentle lysis procedure that should burst the cells, but leave the nuclei intact. Contamination of a relatively RNase-free cytoplasmic environment with nuclear nucleases is thus minimised. Next the polysomes are dissociated with SDS and proteinase K and finally the protein is removed by several phenol/choloroform extractions (1,2).
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