Radiolabeling of DNA using polynucleotide kinase
1Department of Microbiology, The Technical University of Denmark, Lyngby, Denmark.
Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2011
Abstract:
Labeling of DNA fragments at the 5' end with polynucleotide kinase is one of the methods currently employed to obtain highly labeled DNA for sequencing purposes. This end-labeled DNA is used in the partial chemical degradation method for sequencing DNA (see Chapter 51 and ref. 1).
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