Related Experiment Video
Updated: Jun 3, 2026
08:10
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Published on: August 8, 2016
Plasmid screening using single colony lysates.
1Department of Microbiology, University of Surrey, Guildford, Surrey.
Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2011
Summary
This research presents a simple, rapid method for detecting plasmids in bacterial clones and environmental samples using a single colony. The technique is effective for high copy number plasmids and offers a quick screening solution.
Area of Science:
- Molecular Biology
- Microbiology
Background:
- Various methods exist for screening recombinant clones and natural isolates for plasmid presence.
- Existing techniques can be time-consuming or complex.
Purpose of the Study:
- To describe a simple and rapid method for plasmid detection.
- To provide an efficient screening technique for molecular biology and environmental microbiology.
Main Methods:
- The described method is based on Sherratt's technique.
- It utilizes a single bacterial colony directly from an agar plate.
Main Results:
- The method is the simplest and most rapid available for plasmid screening.
- It is particularly effective for high copy number plasmids.
Conclusions:
- This single-colony technique offers an efficient approach to plasmid detection.
- It is applicable to both recombinant clones and environmental isolates.
